After application of testosterone the concentration of testosterone glucuronid increases in the urine remarkable. The testosterone is determined by combined gas-chromatography mass spectrometry with the same analytical procedure as the anabolic steroids excreted in the conjugated fraction. The relation to other endogenous steroids is an important index to state that exogenous testosterone was applied.
The level of epitestosterone the 17-epimere of testosterone does not increase. The relation of testosterone to epitestosterone is normally about 1 in men as well as in women. This quotient increases only when pharmaceutical preparations of testosterone was applied.
Elimination studies with 1.2-dideutero-testosterone show that only a small fraction of the testosterone is converted to epitestosteron